COVID-19 RT-PCR Nucleic Acid Detection Kit (Fluorescent RT-PCR Method)
The RT-PCR nucleic acid detection kit adopts multiple fluorescent RT-PCR technology and uses the conserved regions of the two independent genes "ORF1ab Gene" and "N Gene" of the new coronavirus (COVID-19) as target sites to design specific primers And probes. It is equipped with PCR reaction buffer, reverse transcriptase and DNA polymerase, and uses one-step fluorescent RT-PCR technology to perform in vitro amplification and detection of novel coronavirus (COVID-19) RNA on a fluorescent quantitative PCR instrument to realize the detection of novel coronavirus (COVID-19) RNA. Rapid and accurate diagnosis of Coronavirus (COVID-19). Compared with IgG/IgM antibody detection, RT-PCR nucleic acid detection is more sensitive, but the accuracy and detection rate cannot reach 100%. It has the disadvantage of "False Negative". As the first marker that can be detected after being infected by the new coronavirus (COVID-19), RT-PCR nucleic acid testing is the "Gold Standard" for the detection of new coronavirus (COVID-19).
Compared with IgG/IgM antibody detection, RT-PCR nucleic acid detection takes a longer time, requires the use of related equipment, the detection process is relatively complicated, requires professional training, and the detection cost is higher than antibody detection. In addition, nucleic acid detection kits (fluorescence RT-PCR method) usually use water reagents, which need to be transported and stored at a low temperature of -20°C. Currently, manufacturers use insulation packs + dry ice for transportation. The transportation time needs to be controlled within 7-10 days. Transportation is a huge challenge. If you switch to powder reagents, you can use room temperature transportation, but the sensitivity and accuracy of powder reagents is lower than that of water reagents, and need to be converted to water reagents when used, which places higher demands on operators. In addition, the samples after sampling must be stored and transported at a low temperature (2-8°C), and sent to the laboratory for testing within 48 hours.